human foreskin fibroblast cell line hff1  (Biochrom)

Journal: Alzheimer's & Dementia

Article Title: Interconnections of insulin/IGF‐1 signaling and autophagy abnormalities in Alzheimer's disease

doi: 10.1002/alz.70099

Figure Lengend Snippet: Measuring endocytic and autophagic factors by FICC. A, Schematic of endocytosis (red markers) and autophagy (green markers). B, C, Images from light microscopy (B, upper panels) and corresponding CellProfiler (B, lower panels), or confocal microscopy (C) in HFF1 cells. Images show LC3 and LAMP2 in green and RAB5 and RAB7 in red fluorescence for four different combinations: LC3/RAB5, LC3/RAB7, LAMP2/RAB5, and LAMP2/RAB7. Nuclei were counterstained with DAPI. The insets in (C) depict magnifications from YZ or XZ planes as indicated. Size bars: 5 µm in (B), 20 µm in (C). For visualization purposes, the colors in the CellProfiler images (B) have been amplified. D, CellProfiler data on speckles counts per area and sizes for LC3, LAMP2, RAB5, and RAB7 for naïve AD, OC, and YC cells. Data are from 200 cells per group. One‐way analysis of variance with Sǐdák multiple comparisons was used for group comparisons depicting ns < 0.1; * P < 0.05; ** P < 0.01; *** P < 0.001. AD, late‐onset Alzheimer's disease patient; HFF1, human foreskin fibroblast 1; FICC, fluorescence immunocytochemistry; LAMP2, lysosomal associated membrane protein 2; LC3, microtubule‐associated proteins 1A/1B light chain 3; LOAD, late‐onset Alzheimer's disease; OC, non‐demented age‐matched control; RAB5, Ras related protein 5; RAB7, Ras related protein 7; YC, healthy young control.

Article Snippet: The human foreskin fibroblast 1 (HFF1) cell line was purchased from the American Type Culture Collection (SCRC‐1041).

Techniques: Light Microscopy, Confocal Microscopy, Fluorescence, Amplification, Immunocytochemistry, Membrane, Control

Journal: Molecules

Article Title: From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures

doi: 10.3390/molecules29112472

Figure Lengend Snippet: Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

Article Snippet: The human foreskin fibroblasts 1 (HFF1) cell line was purchased from the American Type Culture Collection (ATCC, LGC Standards S.r.l., Sesto San Giovanni, Italy) and routinely cultured in DMEM supplemented with 1% ( vol / vol ) penicillin and streptomycin, 2 mM L-glutamine, and 10% ( vol / vol ) heat-inactivated FBS (all purchased from Life Technologies).

Techniques: Microscopy, Software, Incubation